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KMID : 0380020090240030253
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 3 p.253 ~ p.258
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Optimization of Fabrication Conditions for Immunosensor Strip to Detect Escherichia coli O157 : H7
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Park So-Jung
Kim Young-Kee
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Abstract
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In this study, the optimization of fabrication conditions was accomplished to make immuno-strip biosensor by the combination of enzyme linked immunosorbent assay (ELISA) and immuno-chromatographic strip techniques for the detection of Escherichia coli O157 : H7. Optimal fabrication conditions of capture antibody concentration, detection antibody concentration, and additive composition of running buffer solution were determined. Optimal concentration was determined as 1.0 mg/mL for both of capture antibody and detection antibody. A composition of 0.5% Tween20 and 3% BSA were selected as optimal additive for buffer solution to prevent non-specific binding.
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KEYWORD
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enzyme-linked immunosorbent assay (ELISA), Escherichia coli O157:H7, immunosensor, optimization
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